Label-free electrochemical analysis of chitosan and glucosamine-containing oligosaccharides. (1st January 2016)

• Record Type: Journal Article
• Authors: Paleček, Emil
• Is Part Of: Electrochimica acta. Volume 187(2016)
• Page Start: 375
• Page End: 380
• Publication Date: 2016-01-01
• Subjects: Chitosan -- Glucosamine-containing oligosaccharides -- Alkaline deacetylation of N-acetylated glycans -- Catalytic hydrogen evolution -- Voltammetry and chronopotentiometry at mercury and solid amalgam electrodes; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2015.11.072
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: Recently we have shown that, in difference to most of polysaccharides, which are considered as electroinactive, chitosan samples containing both glucosamine (GlcN) and N-acetylated glucosamine (GlcNAc) residues are involved in the catalytic hydrogen evolution reaction (CHER) at mercury electrodes, and can be determined by voltammetric or chronopotentiometric stripping. Here we show that polysaccharides and oligosaccharides composed of GlcN, but not those containing only GlcNAc residues, take part in CHER, allowing their easy label-free electrochemical detection. GlcNAc-containing oligosaccharides can be easily transformed into electroactive species by deacetylation using hot KOH treatment. This procedure can be applied also to electroinactive GlcNAc-containing glycans, frequently occurring in glycoproteins, allowing their electrochemical determination at submicromolar level.