Campylobacter jejuni PflB is required for motility and colonisation of the chicken gastrointestinal tract. (December 2015)

• Record Type: Journal Article
• Authors: Kanji, Alpa; Jones, Michael A.; Maskell, Duncan J.; Grant, Andrew J.
• Is Part Of: Microbial pathogenesis. Volume 89(2015)
• Page Start: 93
• Page End: 99
• Publication Date: 2015-12
• Subjects: Campylobacter jejuni -- Flagella -- Motility; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2015.09.010
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. Although the mechanisms by which C. jejuni causes disease are not completely understood, the presence of functional flagella appears to be required for colonisation of the gastrointestinal tract of humans and animals. Therefore much attention has been given to understanding the synthesis and role of flagella in C. jejuni . In this study we report insights into the function of PflB that is essential for Campylobacter motility. We have explored the function of this gene by constructing deletion mutants in C. jejuni strains NCTC11168 and M1, in the genes cj0390 and CJM1_0368, respectively. The mutants were non-motile yet assembled flagella that appeared structurally identical to the wild type. Furthermore the protein is required for C. jejuni colonisation of caeca in a two-week old chicken colonisation model. Highlights: A deletion mutant in pflB was generated in two Campylobacter jejuni strains. C. jejuni pflB mutants were non motile, but still possessed full length flagella. C. jejuni pflB mutants grew faster than the wild type strain. C. jejuni pflB mutants were unable to colonise a 2-week old chicken.