Antibacterial effect of porcine PTX3 against Streptococcus suis type 2 infection. (December 2015)

• Record Type: Journal Article
• Authors: Xu, Jueqiong; Mu, Yanjuan; Zhang, Yue; Dong, Wenyang; Zhu, Yinchu; Ma, Jiale; Song, Wenchao; Pan, Zihao; Lu, Chengping; Yao, Huochun
• Is Part Of: Microbial pathogenesis. Volume 89(2015)
• Page Start: 128
• Page End: 139
• Publication Date: 2015-12
• Subjects: Porcine PTX3 protein -- S. suis 2 -- Eukaryotic expression -- Antibacterial effect; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2015.09.011
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Pentraxin 3 (PTX3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against Streptococcus suis 2 infection. To discover the antibacterial effect of porcine PTX3 against S. suis 2, the 42-kDa PTX3 protein was expressed by Chinese hamster ovary cells (CHO), and an additional eukaryotic expression vector pVAX- ptx3 was constructed. The expressed porcine PTX3 mediated a range of antibacterial activities including increasing phagocytic capacity of primary porcine alveolar macrophages (PAM) against S. suis 2 and inhibiting adhesion of S. suis 2 to human epidermoid cancer cells (Hep-2). In mouse model, pre-intramuscular injecting with pVAX- ptx3 reduced mortality and reduced bacteria loads in blood, spleen, lung and brain compared with that of control group during 2–12 h following intraperitoneal injection ( i.p. ) with S. suis 2. Meanwhile, the expressions of IL-6 and IL-8 in blood were increased in pVAX- ptx3 group, whereas no obvious changes about IL-10. In piglet model, bacteria load in blood of pVAX- ptx3 group was significantly lower than that of control group after i.p. with S. suis 2, correspondingly, expression of IL-6 and IL-8 were significantly increased in pVAX- ptx3 group. In contrast, white blood cell (WBC) and neutrophil cell (NEU) count of peripheral blood in pVAX- ptx3 group were lower than that of control group. These studies described a novel antibacterial role for porcine PTX3 against S. suis 2 both in vitro and in vivo and suggested that porcine PTX3 may be a potential biological agent against S. suis 2 in pig and be used for the clinical prevention and treatment of streptococcosis caused by S. suis 2. Highlights: This study discovered the antibacterial effect of porcine PTX3 against Streptococcus suis 2. The porcine PTX3 increased phagocytic capacity of PAM cell against S. suis 2 and inhibited adhesion of S. suis 2 to Hep-2 cells. Pre-intramuscular injecting with pVAX- ptx3 reduced mortality of mice and reduced bacteria loads in tissues of mice and piglets.