Artocarpus altilis heartwood extract protects skin against UVB in vitro and in vivo. (4th December 2015)

• Record Type: Journal Article
• Authors: Tiraravesit, Narisara; Yakaew, Swanya; Rukchay, Racharat; Luangbudnark, Witoo; Viennet, Celine; Humbert, Philippe; Viyoch, Jarupa
• Is Part Of: Journal of ethnopharmacology. Volume 175(2015)
• Page Start: 153
• Page End: 162
• Publication Date: 2015-12-04
• Subjects: Artocarpin (PubChem CID: 5458461); Artocarpus altilis -- Ultraviolet radiation B (UVB) -- Matrix metalloproteinase-1 (MMP-1) -- Pro-inflammatory cytokine -- Artocarpin-enriched extract; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.09.023
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Artocarpus altilis (Moreceae) has been widely used as a traditional folk medicine in Southeast Asia for the treatment of many diseases, including skin disorders, such as ulcers and dermatitis. Aim of the study: The present study aimed to investigate the ability of an artocarpin-enriched extract to prevent ultraviolet radiation B-induced photodamage. Materials and methods: The content of artocarpin in the extract was determined by high performance liquid chromatography (HPLC). A DPPH assay was used to evaluate the free radical scavenging activity of the extract, which was compared with those ofl -ascorbic acid and α-tocopherol. Cytotoxicity and proliferation of cells treated with the extract were determined using XTT and BrdU assays, respectively. Human skin fibroblasts and keratinocytes were pretreated with the extract for 24 h and later irradiated with ultraviolet radiation B at 128 J/cm 2 . The levels of TNF-α and IL-6 released from ultraviolet radiation B-irradiated keratinocytes and, MMP-1 and type-I procollagen produced by ultraviolet radiation B-irradiated fibroblasts were measured by ELISA and/or western blotting. The hairless skin of male mice (outbred ICR) was treated with the extract orl -ascorbic acid solution prior to exposure to ultraviolet radiation B irradiation. The dose of ultraviolet B irradiation was consecutively increased to 18, 36, 54, and 72 J/cm 2 at weeks 1–4, 4–7, 7–10, and 10–12, respectively. The epidermal thickness and collagen content in the skin of ultraviolet radiation B-irradiated mice were evaluated. Results: The extract concentration of 50 µg/mL was not toxic and did not inhibit the proliferation of fibroblasts. The pretreatment of fibroblasts with 50 µg/mL extract prior to ultraviolet radiation B irradiation attenuated MMP-1 production but did not affect type-I procollagen production. The extract also decreased the ultraviolet radiation B-induced production of TNF-α and IL-6 in keratinocytes. Moreover, the topical administration of the extract suppressed epidermal thickening and collagen loss in chronically ultraviolet radiation B-exposed skin in mice. Conclusions: The experimental study revealed that A. altilis extract suppresses structural alterations in skin damaged by ultraviolet radiation B irradiation. This suppression was, at least partially, mediated by decrease in MMP-1 production in fibroblasts and TNF-α and IL-6 productions in keratinocytes. Graphical abstract: