Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening. (4th December 2015)

• Record Type: Journal Article
• Authors: Duan, Zhen-Zhen; Li, Yu-Hong; Li, Yan-Yan; Fan, Guan-Wei; Chang, Yan-Xu; Yu, Bin; Gao, Xiu-Mei
• Is Part Of: Journal of ethnopharmacology. Volume 175(2015)
• Page Start: 617
• Page End: 625
• Publication Date: 2015-12-04
• Subjects: Ischemia/reoxygenation injury -- H2O2 stress -- Mitochondrial permeability transition pore -- Ca2+ overload -- ROS; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.08.033
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Danhong injection (DHI), a Chinese medical product extracted from Radix et Rhizoma Salviae Miltiorrhizae ( Salvia miltiorrhiza Bge., Labiatae, Danshen in Chinese) and Flos Carthami ( Carthamus tinctorius L., Compositae, Honghua in Chinese), has been widely used for the treatment of ischemic heart disease, and clinical and experimental studies have demonstrated the protective effects against myocardial ischemia/reperfusion injury. Nevertheless, the underlying cellular mechanisms responsible for this protective effect are poorly understood. Aim of the study: The present study aimed to examine the mechanism of DHI in regulating hypoxia/reoxygenation- and H2 O2 -induced cardiomyocytes injury. Materials and methods: Neonatal rat cardiomyocytes were subjected to hypoxia (9 h)-reoxygenation (2 h) or H2 O2 (100 μM) in the presence or absence of DHI (2.5, 5, 10 μg/mL). Intracellular reactive oxygen species (ROS), cytosolic and mitochondrial Ca 2+ concentrations, mitochondrial membrane potential (Δ Ψ m) and mitochondrial permeability transition pore (mPTP) opening were monitored using CMH2 DCFDA, Fluo-4 and rhod-2, JC-1 and calcein, respectively. Cell survival was evaluated using the 2-(4, 5-dimethylthiazol-2-yl)-2, 5 -diphenyltetrazolium bromide (MTT) assay and apoptosis was detected by Annexin V/propidium iodide (PI) staining. Results: DHI improved cell survival following H/R and H2 O2 injury and reduced H/R-induced cytochrome c release and apoptosis when compared with non-DHI treated cells. In addition, DHI attenuated H/R-induced ROS generation, H2 O2 -induced cytosolic and mitochondrial Ca 2+ overload, and cellular ROS generation when compared with H/R- and H2 O2 -only groups. Moreover, DHI significantly inhibited both mPTP opening and Δ Ψ m depolarization. Conclusion: These data demonstrate that the protective mechanism of DHI against H/R- and H2 O2 -induced injury is mediated by the inhibition of mPTP opening via mitigating Ca 2+ overload and ROS generation. Graphical abstract: