The effect of silver or gallium doped titanium against the multidrug resistant Acinetobacter baumannii. (February 2016)

• Record Type: Journal Article
• Authors: Cochis, A.; Azzimonti, B.; Della Valle, C.; De Giglio, E.; Bloise, N.; Visai, L.; Cometa, S.; Rimondini, L.; Chiesa, R.
• Is Part Of: Biomaterials. Volume 80(2016)
• Page Start: 80
• Page End: 95
• Publication Date: 2016-02
• Subjects: Acinetobacter baumannii -- Biofilm -- Gallium -- Silver -- Titanium -- Anodic spark deposition; ASD Anodic Spark Deposition -- AgCis material Antibacterial agent (AgNO3) with chelating agent (l-Cysteine) -- AgNPs material Silver NanoParticles -- GaCis Antibacterial agent (Ga (NO3)3) with chelating agent (l-Cysteine) -- GaOss Antibacterial agent (Ga (NO3)3) with chelating agent (oxalic acid) -- ESKAPE Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp -- MDR Multi Drug Resistant; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2015.11.042
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Implant-related infection of biomaterials is one of the main causes of arthroplasty and osteosynthesis failure. Bacteria, such as the rapidly-emerging Multi Drug Resistant (MDR) pathogen Acinetobacter Baumannii, initiate the infection by adhering to biomaterials and forming a biofilm. Since the implant surface plays a crucial role in early bacterial adhesion phases, titanium was electrochemically modified by an Anodic Spark Deposition (ASD) treatment, developed previously and thought to provide osseo-integrative properties. In this study, the treatment was modified to insert gallium or silver onto the titanium surface, to provide antibacterial properties. The material was characterized morphologically, chemically, and mechanically; biological properties were investigated by direct cytocompatibility assay, Alkaline Phosphatase (ALP) activity, Scanning Electron Microscopy (SEM), and Immunofluorescent (IF) analysis; antibacterial activity was determined by counting Colony Forming Units, and viability assay. The various ASD-treated surfaces showed similar morphology, micrometric pore size, and uniform pore distribution. Of the treatments studied, gallium-doped specimens showed the best ALP synthesis and antibacterial properties. This study demonstrates the possibility of successfully doping the surface of titanium with gallium or silver, using the ASD technique; this approach can provide antibacterial properties and maintain high osseo-integrative potential.