Recognition of purified beta 1, 3/1, 6 glucan and molecular signalling in the intestine of Atlantic salmon. (March 2016)

• Record Type: Journal Article
• Authors: Kiron, Viswanath; Kulkarni, Amod; Dahle, Dalia; Vasanth, Ghana; Lokesh, Jep; Elvebo, Odd
• Is Part Of: Developmental and comparative immunology. Volume 56(2016)
• Page Start: 57
• Page End: 66
• Publication Date: 2016-03
• Subjects: Atlantic salmon -- Beta-1, 3/1, 6 glucan -- MacroGard® -- C-type lectin receptor genes -- Tagln -- Actb -- Psma2; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.11.007
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Atlantic salmon was orally intubated with a highly purified β-glucan product (MacroGard ® ) to study the recognition of the molecule by the receptor genes, the regulation of the downstream signalling genes and global proteins, and the micromorphological changes in the intestine. The β-glucan receptor genes of Atlantic salmon, sclra, sclrb, sclrc and cr3, seem to recognize the molecule, and initiate the downstream ITAM-motif signalling, as evident from the significantly high mRNA levels of ksyk, mapkin2, il1b and mip2a levels. Among the altered proteins, the Apoa4 (involved in carbohydrate and lipid metabolism); Tagln, Actb (uptake of β-glucan); Psma2 (associated with substrate recognition); and Ckt (energy metabolism-related) were the overexpressed ones. The underexpressed proteins included the Uk114, Rpl9, Ctsb and Lgal that are connected to proliferation, LPS-stimulation, Il1b and lactose recognition, respectively. Furthermore, the mRNA levels of igt and the number of immune cells in the distal intestine were found to increase upon β-glucan uptake by the fish. This study provides some clues on the mechanisms by which the β-glucan evokes response in Atlantic salmon, particularly at the intestinal level. Highlights: Recognition and responses of purified β-glucan product at the intestinal level. Upregulation of genes of C-type lectin receptors. Overexpression of proteins linked to uptake and substrate recognition. Presence of more immune cells.