Diffusion-weighted imaging and dynamic contrast-enhanced MRI of experimental breast cancer bone metastases – A correlation study with histology. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Merz, Maximilian; Seyler, Lisa; Bretschi, Maren; Semmler, Wolfhard; Bäuerle, Tobias
• Is Part Of: European journal of radiology. Volume 84:Issue 4(2015)
• Page Start: 623
• Page End: 630
• Publication Date: 2015-04
• Subjects: ADC apparent diffusion coefficient -- a.u. arbitrary units -- DCE-MRI dynamic contrast-enhanced magnetic resonance imaging -- DWI diffusion-weighted imaging -- EORTC European Organization for Research and Treatment of Cancer -- FLASH fast low angle shot -- FOV field of view -- HASTE half-Fourier acquisition single-shot turbo spin echo -- MRI magnetic resonance imaging -- MVC mean vessel caliber -- ROI region of interest -- SMA smooth muscle actin -- T2w MRI T2-weighted magnetic resonance imaging -- TREC recovery time -- TSE turbo spin echo; Diffusion-weighted magnetic resonance imaging -- Dynamic contrast-enhanced magnetic resonance imaging -- Bone metastases -- Angiogenesis -- Brix model -- Breast cancer model; Medical radiology -- Periodicals; Radiology -- Periodicals; Radiologie médicale -- Périodiques; Medical radiology; Periodicals; 616.075705
• DOI: 10.1016/j.ejrad.2015.01.002
• Languages: English
• ISSNs: 0720-048X

Abstract

Abstract: Purpose: To validate imaging parameters from diffusion-weighted imaging and dynamic contrast-enhanced MRI with immunohistology and to non-invasively assess microstructure of experimental breast cancer bone metastases. Materials and methods: Animals bearing breast cancer bone metastases were imaged in a clinical 1.5 T MRI scanner. HASTE sequences were performed to calculate apparent diffusion coefficients. Saturation recovery turbo FLASH sequences were conducted while infusing 0.1 mmol/l Gd–DTPA for dynamic contrast-enhanced MRI to quantify parameters amplitude A and exchange rate constant k ep . After imaging, bone metastases were analyzed immunohistologically. Results: We found correlations of the apparent diffusion coefficients from diffusion-weighted imaging with tumor cellularity as assessed with cell nuclei staining. Histological vessel maturity was correlated negatively with parameters A and k ep from dynamic contrast-enhanced MRI. Tumor size correlated inversely with cell density and vessel permeability as well as positively with mean vessel calibers. Parameters from the rim of bone metastases differed significantly from values of the center. Conclusion: In vivo diffusion-weighted imaging and dynamic contrast-enhanced MRI in experimental bone metastases provide information about tumor cellularity and vascularity and correlate well with immunohistology.