Naringin protects human adipose-derived mesenchymal stem cells against hydrogen peroxide-induced inhibition of osteogenic differentiation. (5th December 2015)

• Record Type: Journal Article
• Authors: Wang, Lei; Zhang, Yu-Ge; Wang, Xiu-Mei; Ma, Long-Fei; Zhang, Yuan-Min
• Is Part Of: Chemico-biological interactions. Volume 242(2015)
• Page Start: 255
• Page End: 261
• Publication Date: 2015-12-05
• Subjects: Osteogenic differentiation -- Oxidative stress -- Naringin -- Wnt signaling -- Human adipose-derived mesenchymal stem cells; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2015.10.010
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Extensive evidence indicates that oxidative stress plays a pivotal role in the development of osteoporosis. We show that naringin, a natural antioxidant and anti-inflammatory compound, effectively protects human adipose-derived mesenchymal stem cells (hADMSCs) against hydrogen peroxide (H2 O2 )-induced inhibition of osteogenic differentiation. Naringin increased viability of hAMDSCs and attenuated H2 O2 -induced cytotoxicity. Naringin also reversed H2 O2 -induced oxidative stress. Oxidative stress induced by H2 O2 inhibits osteogenic differentiation by decreasing alkaline phosphatase (ALP) activity, calcium content and mRNA expression levels of osteogenesis marker genes RUNX2 and OSX in hADMSCs. However, addition of naringin leads to a significant recovery, suggesting the protective effects of naringin against H2 O2 -induced inhibition of osteogenic differentiation. Furthermore, the H2 O2 -induced decrease of protein expressions of β-catenin and clyclin D1, two important transcriptional regulators of Wnt-signaling, was successfully rescued by naringin treatment. Also, in the presence of Wnt inhibitor DKK-1, naringin is no longer effective in stimulating ALP activity, increasing calcium content and mRNA expression levels of RUNX2 and OSX in H2 O2 -exposed hADMSCs. These data clearly demonstrates that naringin protects hADMSCs against oxidative stress-induced inhibition of osteogenic differentiation, which may involve Wnt signaling pathway. Our work suggests that naringin may be a useful addition to the treatment armamentarium for osteoporosis and activation of Wnt signaling may represent attractive therapeutic strategy for the treatment of degenerative disease of bone tissue. Highlights: Naringin protects hADMSCs against H2 O2 -induced inhibition of differentiation. Naringin increased viability of hAMDSCs and attenuated H2 O2 -induced cytotoxicity. Naringin also reversed H2 O2 -induced oxidative stress.