Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets: Application in saccharification of lignocellulose. (January 2016)

• Record Type: Journal Article
• Authors: Xu, Jiaxing; Sheng, Zhenhuan; Wang, Xinfeng; Liu, Xiaoyan; Xia, Jun; Xiong, Peng; He, Bingfang
• Is Part Of: Bioresource technology. Volume 200(2016)
• Page Start: 1060
• Page End: 1064
• Publication Date: 2016-01
• Subjects: Cellulase -- Ionic liquid -- Tolerance -- Graphene oxide -- Saccharification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.10.070
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Design of a functional nanoscale carrier to improve IL tolerance of cellulase. Immobilization of cellulase onto PEGylated graphene oxide nanosheets was achieved. The IL stability of cellulase was enhanced more than 30 times. Rice straw was efficiently hydrolyzed by PEGylated GO-Cellulase in aqueous IL media. The coupling of IL pretreatment and subsequent enzymatic hydrolysis process. Abstract: The objective of the present work was to improve ionic liquid (IL) tolerance of cellulase based on the exploration of functional nanoscale carriers for potential application in lignocellulosic biorefinery. PEGylated graphene oxide (GO) composite was successfully fabricated by chemical binding of 4-arm-PEG-NH2 and GO and applied to the immobilization of cellulase. The PEGylated GO-Cellulase retained 61% of the initial activity in 25% (w/v) 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) while free cellulase only retained 2%. The IL stability was enhanced more than 30 times. The relatively minor change in Km value (from 2.7 to 3.2 mg mL −1 ) after the immobilization suggested that PEGylated GO-Cellulase was capable of closely mimicking the performance of free enzyme. After treating rice straw with [Bmim][Cl] and dilution to a final IL concentration of 15% (w/v), the slurry was directly hydrolyzed using PEGylated GO-Cellulase without IL removing and a high hydrolysis rate of 87% was achieved.