Three new anti-HBV active constituents from the traditional Chinese herb of Yin-Chen (Artemisia scoparia). (24th December 2015)

• Record Type: Journal Article
• Authors: Geng, Chang-An; Huang, Xiao-Yan; Chen, Xing-Long; Ma, Yun-Bao; Rong, Guang-Qing; Zhao, Yong; Zhang, Xue-Mei; Chen, Ji-Jun
• Is Part Of: Journal of ethnopharmacology. Volume 176(2015)
• Page Start: 109
• Page End: 117
• Publication Date: 2015-12-24
• Subjects: Artemisia scoparia (Yin-Chen) -- 4-pyridone glycoside -- Polyacetylenes -- Anti-HBV activity -- LCMS; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.10.032
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Yin-Chen is a famous traditional Chinese medicine (TCM) in China for the treatment of acute and chronic hepatitis. Two species, namely Artemisia scoparia and Artemisia capillaris, are documented in Chinese Pharmacopoeia as the authentic resources for Yin-Chen. Previous investigation has proved that chlorogenic acid analogs and phenolic acids are two main types of the anti-HBV active constituents of A. capillaris . However, there is no investigation concerned with the anti-HBV components of A. scoparia. Aim of the study: The aim of the present study is to recognize the new anti-HBV constituents of A. scoparia by detailed LCMS analyses. Materials and methods: LCMS and bioassay-guided fractionation on the active part of A. scoparia led to the isolation of three new compounds. Their structures were determined by detailed spectroscopic analyses. Anti-HBV assay involving inhibition on HBsAg and HBeAg secretions and HBV DNA replication were performed in virto on HepG 2.2.15 cell line. Results: The 90% ethanol extract of A. scoparia was revealed with anti-HBV activity for the first time, which was further separated into several fractions by column chromatography. Fr. D-4 was revealed with the highest anti-HBV activity, from which three new compounds including one unusual 4-pyridone glucoside (1 ) and two polyacetylene glucosides (2 –3 ) were isolated under the guidance of LCMS analyses. Compounds1 –3 exhibited activity against the secretions of HBsAg and HBeAg, and HBV DNA replication. In particular, compounds2 and3 inhibited HBV DNA replication with IC50 values of 0.07±0.04 and 0.012±0.05 mM, with SI values of 23.6 and 17.1, respectively. Based on the MS/MS experiment, the fragmentation pathways of1 in both positive and negative modes, and2 and3 in negative mode were proposed. The ion pairs of 388–208 (positive) and 432–206 (negative) for1, 503–341 (negative) for2, and 503–203 (negative) for3, could be recognized as their respective diagnostic ions. Conclusions: The first time investigation on the anti-HBV constituents of A. scoparia yielded three new active compounds, which will provide valuable information for understanding the ethnopharmacological usage of Yin-Chen, as well as the chemical difference with A. capillaris . Graphical abstract: