Quality control data of physiological and immunological biomarkers measured in serum and plasma. (November 2015)

• Record Type: Journal Article
• Authors: Jansen, Eugène; Beekhof, Piet; Cremers, Johannes; Weinberger, Birgit; Fiegl, Simone; Toussaint, Olivier; Bernhard, Jürgen; Gonos, Efstathios; Capri, Miriam; Franceschi, Claudio; Sikora, Ewa; Moreno-Villanueva, María; Breusing, Nicolle; Grune, Tilman; Bürkle, Alexander; Dollé, Martijn E.T.
• Is Part Of: Mechanisms of ageing and development. Volume 151(2015)
• Page Start: 54
• Page End: 59
• Publication Date: 2015-11
• Subjects: Biomarkers -- Quality control -- Hemolysis; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2015.06.004
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: In the MARK-AGE project 37 physiological and immunological biomarkers were measured in 3637 serum and plasma samples. The quality of the samples was very good as determined by serum/plasma indices. Only 0.24% of all measurements have to be excluded. The quality control of the biomarker measurements showed a mean inter-assay variation of only 4%. Abstract: In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years. The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples. Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values. A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up. Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.