Anti-adipogenic effect of erucin in early stage of adipogenesis by regulating Ras activity in 3T3-L1 preadipocytes. (December 2015)

• Record Type: Journal Article
• Authors: Chae, Seung Young; Seo, Sang Gwon; Yang, Hee; Yu, Jae Gak; Suk, Su Jin; Jung, Eun Sun; Ji, Hae; Kwon, Jung Yeon; Lee, Hyong Joo; Lee, Ki Won
• Is Part Of: Journal of functional foods. Volume 19:Part A(2016)
• Page Start: 700
• Page End: 709
• Publication Date: 2015-12
• Subjects: AMPK AMP-activated protein kinase -- BCS bovine calf serum -- C/EBP CCAAT/enhancer-binding protein -- ERK extracellular signal-regulated kinase -- FACS fluorescence activated cell sorting -- FAS fatty acid synthase -- FBS fetal bovine serum -- GSK3β glycogen synthase kinase 3β -- HRP horseradish peroxidase -- HSL hormone-sensitive lipase -- IBMX isobutyl methylxanthine -- MCE mitotic clonal expansion -- MEK MAPK/ERK kinase -- MLB Mg-containing lysis buffer -- ORO oil red O -- PPARγ peroxisome proliferator-activated receptors γ -- PVDF polyvinylidene fluoride -- RBD Ras binding domain -- SOS son of sevenless -- SREBP-1c sterol regulatory element-binding protein-1c -- TD terminal differentiation receptors γ -- PVDF polyvinylidene fluoride -- RBD Ras binding domain -- SREBP-1c sterol regulatory element-binding protein-1c -- TD terminal differentiation; Erucin -- Adipogenesis -- Mitotic clonal expansion -- Ras -- 3T3-L1 preadipocytes; Erucin (PubChem CID: 78160); Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.09.060
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Erucin is an active compound in rocket salad and broccoli, used as functional food. Erucin inhibited adipogenesis of 3T3-L1 preadipocytes at early stage of adipogenesis. ERK signalling pathway was involved in anti-adipogenic effect of erucin. Erucin inhibited Ras activity by decreasing GTP-bound Ras protein. Erucin was shown to have potential to be developed as anti-obesity functional food. Abstract: Erucin is a natural isothiocyanate found in rocket salad and broccoli which are believed to have health benefits including weight control effect and used as a functional food globally. Though anti-cancer effect of erucin has been intensively studied, anti-obesity effect of erucin and its molecular mechanisms have not yet been reported. In this study, we investigated anti-adipogenic effect of erucin in 3T3-L1 preadipocytes and its underlying molecular mechanisms. Erucin inhibited adipogenesis of 3T3-L1 preadipocytes and reduced adipogenic gene expressions. This anti-adipogenic activity of erucin was most effective at early stage of adipogenesis including mitotic clonal expansion (MCE). Fluorescence-activated cell sorter (FACS) analysis and trypan blue assay results showed that erucin induced G1/S arrest and inhibited cell proliferation at MCE stage. Western blot analysis results showed that erucin inhibited extracellular signal-regulated kinase (ERK) signaling pathway by blocking Ras activity. Taken together, these results indicated that erucin inhibits adipogenesis of 3T3-L1 preadipocytes by blocking Ras activity at early stage of adipogenesis.