The influence of GAP-43 on orientation of cell division through G proteins. Issue 47 (December 2015)

• Record Type: Journal Article
• Authors: Huang, Rui; Zhao, Junpeng; Ju, Lili; Wen, Yujun; Xu, Qunyuan
• Is Part Of: International journal of developmental neuroscience. Issue 47:Part B(2015:Dec.)
• Page Start: 333
• Page End: 339
• Publication Date: 2015-12
• Subjects: The orientation of cell division -- GAP-43 -- Gαi -- MDCK cells; Developmental neurobiology -- Periodicals; Neurology -- Periodicals; Neurologie du développement -- Périodiques; Developmental neurobiology; Periodicals; 612.8
• DOI: 10.1016/j.ijdevneu.2015.07.013
• Languages: English
• ISSNs: 0736-5748

Abstract

Highlights: The GAP-43 had an influence on the orientation of cell division, determined by cystogenesis in 3D morphogenesis of MDCK cells. The influence of GAP-43 was through its interaction with protein Gαi. The results may explain a role of GAP-43 in neurogenesis. Abstract: Recent studies have shown that GAP-43 is highly expressed in horizontally dividing neural progenitor cells, and G protein complex are required for proper mitotic-spindle orientation of those progenitors in the mammalian developing cortex. In order to verify the hypothesis that GAP-43 may influence the orientation of cell division through interacting with G proteins during neurogenesis, the GAP-43 RNA from adult C57 mouse was cloned into the pEGFP-N1 vector, which was then transfected into Madin–Darby Canine Kidney (MDCK) cells cultured in a three-dimensional (3D) cell culture system. The interaction of GAP-43 with Gαi was detected by co-immunoprecipitation (co-IP), while cystogenesis of 3D morphogenesis of MDCK cells and expression of GAP-43 and Gαi were determined by immunofluorescence and Western blotting. The results showed are as follows: After being transfected by pEGFP-N1-GAP-43, GAP-43 was localized on the cell membrane and co-localized with Gαi, and this dramatically induced a defective cystogenesis in 3D morphogenesis of MDCK cells. The functional interaction between GAP-43 and Gαi proteins was proven by the co-IP assay. It can be considered from the results that the GAP-43 is involved in the orientation of cell division by interacting with Gαi and this should be an important mechanism for neurogenesis in the mammalian brain.