Mass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolica. (1st December 2015)

• Record Type: Journal Article
• Authors: Göçmen, Bayram; Heiss, Paul; Petras, Daniel; Nalbantsoy, Ayse; Süssmuth, Roderich D.
• Is Part Of: Toxicon. Volume 107:Part B(2015)
• Page Start: 163
• Page End: 174
• Publication Date: 2015-12-01
• Subjects: Viperidae -- Anatolian Meadow Viper -- Vipera anatolica -- Snake venomics -- Top-down venomics -- Cytotoxicity -- Bioactivity screening; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2015.09.013
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: This contribution reports on the first characterization of the venom proteome and the bioactivity screening of Vipera anatolica, the Anatolian Meadow Viper. The crude venom as well as an isolated dimeric disintegrin showed remarkable cytotoxic activity against glioblastoma cells. Due to the rare occurrence and the small size of this species only little amount of venom was available, which was profiled by means of a combination of bottom-up and top-down mass spectrometry. From this analysis we identified snake venom metalloproteases, cysteine-rich secretory protein isoforms, a metalloprotease inhibitor, several type A2 phospholipases, disintegrins, a snake venom serine protease, a C-type lectin and a Kunitz-type protease inhibitor. Furthermore, we detected several isoforms of above mentioned proteins as well as previously unknown proteins, indicating an extensive complexity of the venom which would have remained undetected with conventional venomic approaches. Graphical abstract: