A reference gene set for chemosensory receptor genes of Manduca sexta. (November 2015)

• Record Type: Journal Article
• Authors: Koenig, Christopher; Hirsh, Ariana; Bucks, Sascha; Klinner, Christian; Vogel, Heiko; Shukla, Aditi; Mansfield, Jennifer H.; Morton, Brian; Hansson, Bill S.; Grosse-Wilde, Ewald
• Is Part Of: Insect biochemistry and molecular biology. Volume 66(2015:Nov.)
• Page Start: 51
• Page End: 63
• Publication Date: 2015-11
• Subjects: Olfaction -- Taste -- Lepidoptera -- Ionotropic receptor -- Pheromone receptor -- Gustatory receptor; GR gustatory receptor -- OR olfactory receptor -- IR ionotropic receptor -- OSN olfactory sensory neuron -- ORCo olfactory receptor coreceptor -- OGS official gene set -- RPKM reads per kilo base per million mapped reads -- SR sugar receptor; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2015.09.007
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta . Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context, mainly the interaction with its host plants. In recent years transcriptomic data has led to the discovery of members of all major chemosensory receptor families in the species, but the data was fragmentary and incomplete. Here we present the analysis of the newly available high-quality genome data for the species, supplemented by additional transcriptome data to generate a high quality reference gene set for the three major chemosensory receptor gene families, the gustatory (GR), olfactory (OR) and antennal ionotropic receptors (IR). Coupled with gene expression analysis our approach allows association of specific receptor types and behaviors, like pheromone and host detection. The dataset will provide valuable support for future analysis of these essential chemosensory modalities in this species and in Lepidoptera in general. Graphical abstract: Highlights: The OR repertoire of M. sexta comprises 73 genes. We report additional sex specific ORs; one female and one male specific one. We identified 21 IR candidates in the genome. M. sexta does not possess an expansion of sugar receptors but an expansion of bitter receptors.