Validating multiplexes for use in conjunction with modern interpretation strategies. (January 2016)

• Record Type: Journal Article
• Authors: Taylor, Duncan; Bright, Jo-Anne; McGoven, Catherine; Hefford, Christopher; Kalafut, Tim; Buckleton, John
• Is Part Of: Forensic science international. Volume 20(2016:Jan.)
• Page Start: 6
• Page End: 19
• Publication Date: 2016-01
• Subjects: Forensic DNA interpretation -- GlobalFiler -- Continuous DNA interpretation -- STRmix -- Modelling -- DNA mixtures; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2015.09.011
• Languages: English
• ISSNs: 1872-4973

Abstract

Abstract: In response to requests from the forensic community, commercial companies are generating larger, more sensitive, and more discriminating STR multiplexes. These multiplexes are now applied to a wider range of samples including complex multi-person mixtures. In parallel there is an overdue reappraisal of profile interpretation methodology. Aspects of this reappraisal include 1. The need for a quantitative understanding of allele and stutter peak heights and their variability, 2. An interest in reassessing the utility of smaller peaks below the often used analytical threshold, 3. A need to understand not just the occurrence of peak drop-in but also the height distribution of such peaks, and 4. A need to understand the limitations of the multiplex-interpretation strategy pair implemented. In this work we present a full scheme for validation of a new multiplex that is suitable for informing modern interpretation practice. We predominantly use GlobalFiler™ as an example multiplex but we suggest that the aspects investigated here are fundamental to introducing any multiplex in the modern interpretation environment.