Proinflammatory cytokine interleukin-1β suppresses cold-induced thermogenesis in adipocytes. (January 2016)

• Record Type: Journal Article
• Authors: Goto, Tsuyoshi; Naknukool, Supaporn; Yoshitake, Rieko; Hanafusa, Yuki; Tokiwa, Soshi; Li, Yongjia; Sakamoto, Tomoya; Nitta, Takahiro; Kim, Minji; Takahashi, Nobuyuki; Yu, Rina; Daiyasu, Hiromi; Seno, Shigeto; Matsuda, Hideo; Kawada, Teruo
• Is Part Of: Cytokine. Volume 77(2016)
• Page Start: 107
• Page End: 114
• Publication Date: 2016-01
• Subjects: IL-1β interlekine-1β -- UCP1 uncoupling protein 1 -- ERK extracellular signal-regulated kinase -- MAPK mitogen-activated protein kinase -- JNK c-jun N-terminal kinase -- LPS lipopolysaccharide -- WAT white adipose tissue -- BAT brown adipose tissue -- NLRP3 nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 -- ASC apoptosis-associated speck-like protein containing carboxy-terminal caspase activation recruitment domain -- HFD high-fat diet -- cAMP cyclic adenosine monophosphate -- CRE cAMP response element -- CREB CRE binding protein -- ATF2 activating transcription factor-2 -- SV stromal-vascular -- TNF-α tumor necrosis factor-α -- MCP-1 monocyte chemoattractant protein-1 -- NO nitric oxide -- IL-1Ra IL-1 receptor antagonist -- iNOS inducible NO synthase -- ISO isoproterenol; Obesity -- Brown-like adipocyte -- Brown adipocyte -- UCP1 -- Inflammation -- IL-1β; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2015.11.001
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: IL-1β expression levels were enhanced in white adipose tissue from obese mice. IL-1β attenuated UCP1 induction via ERK activation in C3H10T1/2 adipocytes. IL-1β impaired β-adrenoreceptor-stimulated activation of the UCP1 promoter. IL-1β administration impaired cold-induced UCP1 upregulation in adipose tissues. Abstract: In this study, we investigated the effects of interleukin-1β (IL-1β), a typical proinflammatory cytokine on the β-adrenoreceptor-stimulated induction of uncoupling protein 1 (UCP1) expression in adipocytes. IL-1β mRNA expression levels were upregulated in white adipose tissues of obese mice and in RAW264.7 macrophages under conditions designed to mimic obese adipose tissue. Isoproterenol-stimulated induction of UCP1 mRNA expression was significantly inhibited in C3H10T1/2 adipocytes by conditioned medium from lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison with control conditioned medium. This inhibition was significantly attenuated in the presence of recombinant IL-1 receptor antagonist and IL-1β antibody, suggesting that activated macrophage-derived IL-1β is an important cytokine for inhibition of β-adrenoreceptor-stimulated UCP1 induction in adipocytes. IL-1β suppressed isoproterenol-induced UCP1 mRNA expression in C3H10T1/2 adipocytes, and this effect was partially but significantly abrogated by inhibition of extracellular signal-regulated kinase (ERK). IL-1β also suppressed the isoproterenol-induced activation of the UCP1 promoter and transcription factors binding to the cAMP response element. Moreover, intraperitoneal administration of IL-1β suppressed cold-induced UCP1 expression in adipose tissues. These findings suggest that IL-1β upregulated in obese adipose tissues suppresses β-adrenoreceptor-stimulated induction of UCP1 expression through ERK activation in adipocytes.