Stepwise ethanolic precipitation of sugar beet pectins from the acidic extract. (20th January 2016)

• Record Type: Journal Article
• Authors: Guo, Xiaoming; Meng, Hecheng; Zhu, Siming; Tang, Qiang; Pan, Runquan; Yu, Shujuan
• Is Part Of: Carbohydrate polymers. Volume 136(2016)
• Page Start: 316
• Page End: 321
• Publication Date: 2016-01-20
• Subjects: Sugar beet pectins -- Ethanolic precipitation -- Fractionation -- Characteristics; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.09.003
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A stepwise ethanolic precipitation procedure was developed. Sugar beet pectins were fractionated by the stepwise ethanolic precipitation. Fractions with different chemical and surface features were produced. Abstract: A stepwise ethanol-precipitation (SEP) procedure was developed for the purification of sugar beet pectins (SBP) from a pectin-containing aqueous extract. Five fractions of different chemical and molecular characteristics were produced by stepwise elevating the alcohol concentration of the precipitation medium from 50% to 80% v/v. Comparison of chemical and macromolecular features between the obtained fractions indirectly suggested that the ability of pectin to solubilize in the ethanol-water binary mixture depended greatly on the polymer structure. Fractions rich in neutral sugars were precipitated at relatively high ethanol concentrations, probably due to the enhanced interactions generated between pectin side chains and solvent molecules. Furthermore, the obtained fractions displayed different surface activities. Results obtained in this work indicate that the SEP procedure is more selective with respect to pectin structural features and surface properties than the one-step ethanolic precipitation.