Cell mechanosensory recognizes ligand compliance at biomaterial interface. (January 2016)
- Record Type:
- Journal Article
- Title:
- Cell mechanosensory recognizes ligand compliance at biomaterial interface. (January 2016)
- Main Title:
- Cell mechanosensory recognizes ligand compliance at biomaterial interface
- Authors:
- Cosenza, Chiara
Lettera, Vincenzo
Causa, Filippo
Scognamiglio, Pasqualina Liana
Battista, Edmondo
Netti, Paolo Antonio - Abstract:
- Abstract: Cells activate signalling through ligand-receptor bonds by sensing the mechanical properties of the surrounding extracellular matrix (ECM). Ligands, indeed, have to withstand the pulling force elicited by cell receptors through focal adhesions (FAs). On this basis, we developed functional ligands to be simply adsorbed on surfaces and constituted by a two-domain peptide: one derived from ECM proteins and available to receptors to offer biochemical cues, and another adsorbed on material to withstand the tension upon receptor engagement. Tuneable compliance of the anchoring domain of the peptide ligand was verified by single peptide analysis through molecular dynamics and adsorption measurements. We showed that the highest adsorbed peptides combined with integrin cell-binding motifs allow for the cell recognition and polarization with larger mature FA areas. On the contrary, the lowest adsorbed sequences did not provide mechanical resistance to the integrin pulling action, leading to more rounded cells with smaller FA areas. This evidence demonstrates that cell mechanosensory can discriminate ligands on surfaces and should be considered as a criterion in ligand design for material bioactivation.
- Is Part Of:
- Biomaterials. Volume 76(2016)
- Journal:
- Biomaterials
- Issue:
- Volume 76(2016)
- Issue Display:
- Volume 76, Issue 2016 (2016)
- Year:
- 2016
- Volume:
- 76
- Issue:
- 2016
- Issue Sort Value:
- 2016-0076-2016-0000
- Page Start:
- 282
- Page End:
- 291
- Publication Date:
- 2016-01
- Subjects:
- Ligand -- Material bioactivation -- Peptide -- Mechanosensory
Biomedical materials -- Periodicals
Biocompatible Materials -- Periodicals
Biomatériaux -- Périodiques
610.28 - Journal URLs:
- http://www.sciencedirect.com/science/journal/01429612 ↗
http://www.clinicalkey.com/dura/browse/journalIssue/01429612 ↗
http://www.clinicalkey.com.au/dura/browse/journalIssue/01429612 ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.biomaterials.2015.10.068 ↗
- Languages:
- English
- ISSNs:
- 0142-9612
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 2087.715000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 1000.xml