Differences between calcium-stimulated and storage-induced erythrocyte-derived microvesicles. Issue 2 (October 2015)

• Record Type: Journal Article
• Authors: Prudent, Michel; Crettaz, David; Delobel, Julien; Seghatchian, Jerard; Tissot, Jean-Daniel; Lion, Niels
• Is Part Of: Transfusion and apheresis science. Volume 53:Issue 2(2015)
• Page Start: 153
• Page End: 158
• Publication Date: 2015-10
• Subjects: Calcium -- Erythrocyte -- Microvesicle -- Proteomics -- Storage -- Transfusion; Blood -- Transfusion -- Periodicals; Hemapheresis -- Periodicals; 615.39
• DOI: 10.1016/j.transci.2015.10.012
• Languages: English
• ISSNs: 1473-0502

Abstract

Abstract: Microvesicles (MVs), or microparticles, are a complex, dynamic and functional part of cells. Red blood cell (RBC)-derived MVs are naturally produced in vivo (during normal aging processes or in several diseases) as well as ex vivo during cold storage of RBCs, or in vitro by ATP depletion or treatment with Ca 2+ and calcium ionophore. All these MVs are equivalently classified according to their size and/or surface markers. Nevertheless, their content in proteins can differ and a few differences in terms of lipid raft proteins, notably stomatin and flotillin-2, have been reported. Based on two-dimensional gel electrophoreses, the present study highlights the differences between MVs induced during storage of RBCs (storage-MVs) and MVs stimulated by Ca 2+ entry (Ca-MVs). Upon treatment, Ca-MVs are formed following a clear recruitment of Ca 2+ -binding proteins (sorcin, grancalcin, PDCD6) and particularly annexins (4 and 5). Therefore, it emerges that different molecular pathways are available to produce similar MVs by disturbing the membrane/cytoskeleton interactions. Interestingly, these differences provide non-negligible pieces of information on the parent cells, and the mechanisms and modes of actions involved in the formation of MVs. In addition to biophysical characterization, protein analysis is important to classify these cellular corpuscles and evaluate their potential impacts in diseases or transfusion medicine.