A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization. Issue 12 (December 2015)

• Record Type: Journal Article
• Authors: Tian, Xueli; Wang, Yanfang; Zhao, Fengying; Liu, Jinlin; Yin, Jun; Chen, Dieyan; Ma, Wanyun; Ke, Xiaoyan
• Is Part Of: Leukemia research. Volume 39:Issue 12(2015:Dec.)
• Page Start: 1414
• Page End: 1420
• Publication Date: 2015-12
• Subjects: 3D-FISH -- Acute myeloid leukemia -- Chromosome pair -- Translocation -- Prognosis; Leukemia -- Periodicals; Leukemia -- Periodicals; Leucémie -- Périodiques; Leukemia; Periodicals; Electronic journals; Electronic journals; 616.9941905
• DOI: 10.1016/j.leukres.2015.09.013
• Languages: English
• ISSNs: 0145-2126

Abstract

Highlights: We studied spatial organizations of chromosome 8 and 21 for AML-M2 by 3D-FISH. Nuclei were classified into normal, transitional, and malignant nuclei. Occurrences of each type nuclei were associated with the disease progression. d / R of chromosome 8 was similar to d / R of chromosome 21 for relapse sample. Centers of close chromosomal pairs were more internal than normal pairs. Abstract: Interphase heterogenous chromosomes spatially close to each other are predominantly located near the center of nuclei and are prone to incur translocations. We screened a t(8;21) (q22;q22) acute myeloid leukemia-M2 patient during three phases (post-chemotherapy, remittent stage, and relapse) and a donor of normal karyotype as control by two-(2D) and three-dimensional (3D)-fluorescence in situ hybridization (FISH). Our classification of nuclei (normal, transitional, and malignant nuclei) by 3D-FISH analyses may provide a more precise prognosis than 2D-FISH results, especially for remittent stage sample in our study, in which 2D-FISH findings showed normal results, whereas 3D-FISH results showed extreme abnormalities (normal nuclei 27%, transitional nuclei 36%, malignant nuclei 37%). The relative radial positions ( d / R ) of chromosomes 8 were similar to d / R of chromosomes 21 for the relapse sample. We classified heterogenous chromosome pairs into close pairs and normal pairs based on their relative distances ( d′ /(2 R )). The centers of close pairs were more internal than normal pairs in nuclei in all samples, and the d / R values of a given-type pairwise heterogenous chromosomes were similar among four samples. Our data demonstrate that the classification of nuclei based on spatial organization of chromosomes by 3D-FISH is reasonable and essential for evaluating acute myeloid leukemia prognosis.