PH responsive translocation of an anticancer drug between cyclodextrin and DNA. Issue 119 (17th November 2015)

• Record Type: Journal Article
• Authors: Gavvala, Krishna; Satpathi, Sagar; Hazra, Partha
• Is Part Of: RSC advances. Volume 5:Issue 119(2015)
• Page Start: 98080
• Page End: 98086
• Publication Date: 2015-11-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra19839a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Herein, the pH triggered translocation of EPT in between γ-cyclodextrin and DNA is monitored using fluorescence switch of the drug. Abstract : Ellipticine, a well known anticancer drug, emits intense green color when it is intercalated in DNA. It exhibits blue color inside the nano-cavity of a supramolecular host, γ-cyclodextrin (γ-CD). Inspired by these unique fluorescence switching properties of the anticancer drug, in the present work we have monitored the interplay of the drug between γ-CD and DNA by varying the medium pH as a stimulus. Here, steady-state and picosecond time-resolved fluorescence as well as circular dichroism techniques are employed to decipher the location of the drug inside the γ-CD nano-cavity and DNA. Our results confirm that at higher pH the drug selectively stays at γ-CD, even in the presence of biopolymers and exhibits blue color; whereas at lower pH, it is preferentially located in DNA even in the presence of γ-CD and emits a green color. We believe this kind of pH driven translocation of drugs monitored by fluorescence switching may find possible applications in controlled release of the drug inside cells.