Expression of adhesion proteins (E-cadherin and β-catenin) and cell proliferation (Ki-67) at the invasive tumor front in conventional oral squamous cell and basaloid squamous cell carcinomas. (January 2016)

• Record Type: Journal Article
• Authors: Pereira, Carlos Henrique; Morais, Marília Oliveira; Martins, Allisson Filipe Lopes; Soares, Mariana Quirino Silveira; Alencar, Rita de Cássia Gonçalves; Batista, Aline Carvalho; Leles, Cláudio Rodrigues; Mendonça, Elismauro Francisco
• Is Part Of: Archives of oral biology. Volume 61(2016)
• Page Start: 8
• Page End: 15
• Publication Date: 2016-01
• Subjects: SCC squamous cell carcinoma -- BSCC basaloid squamous cell carcinoma -- WHO World Health Organization -- E-cad E-cadherin -- β-cat β-catenin -- ITF invasive tumor front -- EMT epithelial mesenchymal transition -- HE hematoxylin and eosin -- PBS phosphate buffer solution -- CI confidence interval -- HPV human papillomavirus; Squamous cell carcinoma -- Basaloid squamous cell carcinoma -- Ki-67 protein -- E-cadherin -- β-catenin -- Front invasion tumoral; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2015.10.003
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: E-cad, B-Cat and Ki-67 were investigated in oral SCC and BSCC. There is no difference of expression of B-cat and E-cad in oral SCC and BSCC. Lower expression of E-Cad and B-cat in cytoplasmic membrane and high in cytoplasm. Ki-67 expression has no significance in the ITF of SCC and BSCC in oral cavity. There is no significant difference between these lesions in oral cavity. Abstract: Objective: Investigate, on a comparative basis, the expression of the adhesion molecules E-cadherin (E-cad), β-catenin (β-cat) and the proliferation index (Ki-67) at the invasive tumor front (ITF) in squamous cell carcinoma (SCC) and basaloid squamous cell carcinoma (BSCC). Material and methods: Thirty-five SCC and 16 BSCC cases were evaluated by immunohistochemistry. Clinicopathological and survival data were also evaluated and compared. Results: There was a low expression of E-cad in the cytoplasmic membrane ( p = 0.50) as well as in the nucleus ( p = 0.31) for both SCC and BSCC. A high expression of E-cad was seen in the cytoplasm for the SCC group (80%) when compared to the BSCC group (25%) ( p < 0.01). The expression of β-cat was low in the cytoplasmic membrane and high in the cytoplasm in both SCC and BSCC groups. Both types of carcinoma presented low expressions of β-cat in the nucleus ( p = 0.03). The Ki-67 expression was low irrespective of tumor variant. The high expression of E-cad in the cytoplasm was associated with T3/T4 tumors ( p = 0.04) in the SCC group and there was no significant association of E-cad, β-cat, Ki-67 with the other clinical variables. In terms of disease-free survival and overall survival, there were no significant differences between SCC and BSCC. Conclusion: The E-cad–β-cat system was found to be dysregulated in both oral SCC and oral BSCC. The Ki-67 cell proliferation index was extremely low in the cases investigated and consequently had no prognostic value.