Determination of fusaric acid in maize using molecularly imprinted SPE clean‐up. Issue 3 (10th December 2013)

• Record Type: Journal Article
• Authors: Appell, Michael; Jackson, Michael A.; Wang, Lijuan C.; Ho, Che‐Hin; Mueller, Anja
• Is Part Of: Journal of separation science. Volume 37:Issue 3(2014:Feb.)
• Page Start: 281
• Page End: 286
• Publication Date: 2013-12-10
• Subjects: Food safety -- HPLC -- Ion pair chromatography -- Molecularly imprinted polymers -- Mycotoxins; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201301065
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean‐up using mimic‐templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2‐dimethylaminoethyl methacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion‐pairing chromatography with diode‐array UV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1–100 μg/g with recoveries between 83.9 and 92.1%.