Reprint of: Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro. (November 2015)

• Record Type: Journal Article
• Authors: Tomankova, Katerina; Horakova, Jana; Harvanova, Monika; Malina, Lukas; Soukupova, Jana; Hradilova, Sarka; Kejlova, Kristina; Malohlava, Jakub; Licman, Libor; Dvorakova, Marketa; Jirova, Dagmar; Kolarova, Hana
• Is Part Of: Food and chemical toxicology. Volume 85(2015)
• Page Start: 20
• Page End: 30
• Publication Date: 2015-11
• Subjects: Silver nanoparticles -- Titanium dioxide nanoparticles -- In vitro cytotoxicity -- Raman spectroscopy -- Cell uptake; ROS reactive oxygen species -- MMP mitochondrial membrane potential -- DMEM Dulbecco's modified Eagle's medium -- FBS foetal bovine serum -- IC50 50% lethal concentration -- NP nanoparticle -- DLS dynamic light scattering -- AAS atomic absorption spectroscopy -- AFM atomic force microscopy -- KMCA k-mean cluster analysis -- RT room temperature; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2015.10.012
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage. Highlights: Evaluation of the declared parameters of commercially available TiO2 NPs and Ag NPs. Data provided by the producers do not reflect reality. All of the studied NPs caused significant DNA damage and cell apoptosis. All the tested NPs penetrated into the SVK14 cells.