Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lactobacillus agilis CCUG31450. Issue 110 (23rd October 2015)

• Record Type: Journal Article
• Authors: Gudiña, Eduardo J.; Fernandes, Elisabete C.; Teixeira, José A.; Rodrigues, Lígia R.
• Is Part Of: RSC advances. Volume 5:Issue 110(2015)
• Page Start: 90960
• Page End: 90968
• Publication Date: 2015-10-23
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra11659g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Lactobacillus agilis CCUG31450 produces a cell-bound biosurfactant (glycoprotein) which exhibits anti-adhesive and antimicrobial activities against Staphylococcus aureus . Abstract : In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN m −1, and exhibited a high emulsifying activity ( E 24 = 60%). The amount of biosurfactant produced was 84 mg l −1, with a cmc of 7.5 mg ml −1 . A preliminary chemical characterization by Fourier transform infrared spectroscopy indicated that the biosurfactant is a glycoprotein. Using cheese whey as an alternative culture medium, biosurfactant production was increased up to 960 mg l −1 . The biosurfactant exhibited a considerable anti-adhesive activity against Staphylococcus aureus, as well as antimicrobial activity against S. aureus, Streptococcus agalactiae and Pseudomonas aeruginosa . This study represents the first description of a biosurfactant produced by a L. agilis strain. The results obtained open future prospects for the application of this biosurfactant to reduce or inhibit the adhesion of pathogenic microorganisms (such as S. aureus ) in several biomedical applications.