Physico-chemical characterization of asolectin–genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability. (December 2015)

• Record Type: Journal Article
• Authors: Lopes de Azambuja, Carla Roberta; dos Santos, Lurdiane Gomes; Rodrigues, Marisa Raquel; Rodrigues, Renan Ferreira Meneses; da Silveira, Elita Ferreira; Azambuja, Juliana Hofstatter; Flores, Alex F.C.; Horn, Ana Paula; Dora, Cristiana Lima; Muccillo-Baisch, Ana Luisa; Braganhol, Elizandra; da Silva Pinto, Luciano; Parize, Alexandre Luís; de Lima, Vânia Rodrigues
• Is Part Of: Chemistry and physics of lipids. Volume 193:(2015)
• Page Start: 24
• Page End: 35
• Publication Date: 2015-12
• Subjects: ASO soybean asolectin -- DLS dynamic light scattering -- DMPC dimyristoylphosphatidylcholine -- DPPC dipalmitoylphosphatidylcholine -- DSC differential scanning calorimetry -- DMEM Dulbecco's modified Eagle's medium -- ΔH enthalpy variation -- FBS fetal bovine serum -- FID free induction decay -- FTIR Fourier transform infrared spectroscopy -- HATR–FTIR horizontal attenuated total reflection–Fourier transform infrared spectroscopy -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- NMR nuclear magnetic resonance -- OH hydroxyl radical -- S.D. standard deviation -- TBARS thiobarbituric acid reactive substances -- TSP sodium 3-(trimethylsilyl)-[2, 2, 3, 3-2H4]-1-propionate -- τ time delay -- ν stretching vibration; Genistein -- Asolectin liposomes -- DSC -- NMR -- Spectroscopy study; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipids; Periodicals; Electronic journals; 547.77
• DOI: 10.1016/j.chemphyslip.2015.10.001
• Languages: English
• ISSNs: 0009-3084

Abstract

Graphical abstract: Highlights: Genistein–asolectin liposomes interaction was studied by several techniques. Genistein effects on liposomes were correlated with its biological activities. Genistein orders lipid phosphate, carbonyl and acyl chain methylenes regions. Genistein inhibits lipid peroxidation induced by hydroxyl radical. Liposome-loaded genistein favored the antitumor effect against glioma cells lines. Abstract: In this study, the interaction between soy isoflavone genistein and asolectin liposomes was investigated by monitoring the effects of isoflavone on lipidic hydration, mobility, location and order. These properties were analyzed by the following techniques: horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), low-field 1 H nuclear magnetic resonance (NMR), high-field 31 P NMR, zeta potential, differential scanning calorimetry (DSC) and UV–vis spectroscopy. The antioxidant and antitumoral activities of the genistein liposomal system were also studied. The genistein saturation concentration in ASO liposomes corresponded to 484 μM. HATR–FTIR results indicated that genistein influences the dynamics of the lipidic phosphate, choline, carbonyl and acyl chain methylenes groups. At the lipid polar head, HATR–FTIR and 31 P NMR results showed that the isoflavone reduces the hydration degree of the phosphate group, as well as its mobility. Genistein ordered the lipid interfacial carbonyl group, as evidenced by the HATR–FTIR bandwidth analysis. This ordering effect was also observed in the lipidic hydrophobic region, by HATR–FTIR, NMR, DSC and turbidity responses. At the saturation concentration, liposome-loaded genistein inhibits the lipid peroxidation induced by hydroxyl radical in 90.9%. ASO liposome-loaded genistein at 100 μM decreased C6 glioma cell viability by 57% after 72 h of treatment. Results showed an increase of the genistein in vitro activities after its incorporation in liposomes. The data described in this work will contribute to a better understanding of the interaction between genistein and a natural-source membrane and of its influence on isoflavone biological activities. Furthermore, the antitumoral results showed that genistein-based liposomes, which contain natural-sourced lipids, may be promising as a drug delivery system to be used in the glioma therapy.