Novel sulfated phosphoglycolipids from Natronomonas moolapensis. (October 2015)

• Record Type: Journal Article
• Authors: Hoffmann, Andreas; Kovermann, Michael; Oberwinkler, Tanja; Siedler, Frank; Cortina, Niña Socorro; Balbach, Jochen; Oesterhelt, Dieter
• Is Part Of: Chemistry and physics of lipids. Volume 191:(2015)
• Page Start: 8
• Page End: 15
• Publication Date: 2015-10
• Subjects: BR bacteriorhodopsin -- DEPT distortionless enhancement by polarization transfer -- DMF dimethylformamide -- HMBC heteronuclear multiple bond coherence -- HSQC heteronuclear single quantum coherence -- MWCO molecular weight cut-off -- NOE(SY) nuclear overhauser effect (spectroscopy) -- PA phosphatidic acid -- PG phosphatidylglycerol -- PGS phosphatidylglycerol sulfate -- PHA polyhydroxyalkanoic acids -- S-DGA-3 sulfated diglycosylarchaeol-3 -- S-TGA-1 sulfated triglycosylarchaeol-1 -- TOCSY total correlation spectroscopy; Natronomonas moolapensis strain 8.8.11T -- Halophilic archaea -- Phosphatidylglycerol -- Mass -- spectrometry -- NMR; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipids; Periodicals; Electronic journals; 547.77
• DOI: 10.1016/j.chemphyslip.2015.06.004
• Languages: English
• ISSNs: 0009-3084

Abstract

Highlights: Uncharacterized glycolipid species from the halophilic archaeon Natronomonas moolapensis were identified and purified. An unusual glycolipid core structure based on phosphatidylglycerol was identified by MS and NMR. Two mono-glycosyl moieties, d -glucopyranose-6-sulfate andd -galactofuranose-6-sulfate, respectively, were identified. Abstract: Polar lipid pattern determination is often used for the taxonomic classification of halophilic Archaea in addition to a genomic characterization. During the analysis of polar lipid extracts from the recently described haloarchaeon Natrononomonas moolapensis, an unknown glycolipid was detected. Fragmentation patterns observed from preliminary mass spectrometric analysis initially suggested the presence of a sulfo-hexosyl-phosphatidylglycerol. However, by NMR spectroscopy and enzymatic assays the existence of two isomeric molecules with different hexoses (1-(6-sulfo-d -glcp/galf-β1, 2-glycero)-phospho-2, 3-diphytanylglycerol) could be shown. The structural origin from phosphatidylglycerol distinguishes these glycolipids within Archaea, because all other characterized haloarchaeal glycolipids consist of diphytanylglycerol directly linked to an oligoglycosyl moiety. Now the door is open to investigate the physical and functional consequences of these architectural differences of the head groups.