Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. (January 2016)

• Record Type: Journal Article
• Authors: Cheng, Zhuan; Jiang, Jiaqi; Wu, Hui; Li, Zhimin; Ye, Qin
• Is Part Of: Bioresource technology. Volume 200(2016)
• Page Start: 897
• Page End: 904
• Publication Date: 2016-01
• Subjects: 3-Hydroxypropionate -- Acetyl-CoA carboxylase -- Malonyl-CoA reductase -- Corynebacterium glutamicum -- Escherichia coli; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.10.107
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: 3-Hydroxypropionate can be biosynthesized by engineered E. coli efficiently from glucose. The Acc of C. glutamicum and MCR of C. aurantiacus were co-overexpressed in E. coli . The concentration of 3-HP achieved 10.08 g/L in 36 h. Abstract: In this study, production of 3-HP via malonyl-CoA was investigated by using metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus . Three engineered E. coli strains with different host-vector systems were constructed and investigated. The results indicated that the combination of E. coli BL21(DE3) and pET28a was the most efficient host-vector system for 3-HP production, and the highest concentration of 3-HP attained in shake flask cultivation reached 1.80 g/L by the strain BE-MDA with induction at 0.25 mM IPTG and 25 °C, and supplementation of NaHCO3 and biotin. In fed-batch fermentation performed in a 5-L reactor, the concentration of 3-HP achieved 10.08 g/L in 36 h.