Global transcriptional responses of Acidithiobacillus ferrooxidans Wenelen under different sulfide minerals. (January 2016)

• Record Type: Journal Article
• Authors: Latorre, Mauricio; Ehrenfeld, Nicole; Cortés, María Paz; Travisany, Dante; Budinich, Marko; Aravena, Andrés; González, Mauricio; Bobadilla-Fazzini, Roberto A.; Parada, Pilar; Maass, Alejandro
• Is Part Of: Bioresource technology. Volume 200(2016)
• Page Start: 29
• Page End: 34
• Publication Date: 2016-01
• Subjects: Acidithiobacillus ferrooxidans -- Microarrays -- Sulfur minerals -- Copper resistance -- Bioleaching; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.09.110
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: At. ferrooxidans Wenelen is a native strain isolated from a copper mine. Transcriptional response of Wenelen under sulfide minerals was studied. Sulfur metabolism and Cu resistance genes were induced. Wenelen is currently used in a large-scale bioleaching bioreactor. Abstract: In order to provide new information about the adaptation of Acidithiobacillus ferrooxidans during the bioleaching process, the current analysis presents the first report of the global transcriptional response of the native copper mine strain Wenelen (DSM 16786) oxidized under different sulfide minerals. Microarrays were used to measure the response of At. ferrooxidans Wenelen to shifts from iron supplemented liquid cultures (reference state) to the addition of solid substrates enriched in pyrite or chalcopyrite. Genes encoding for energy metabolism showed a similar transcriptional profile for the two sulfide minerals. Interestingly, four operons related to sulfur metabolism were over-expressed during growth on a reduced sulfur source. Genes associated with metal tolerance (RND and ATPases type P) were up-regulated in the presence of pyrite or chalcopyrite. These results suggest that At. ferrooxidans Wenelen presents an efficient transcriptional system developed to respond to environmental conditions, namely the ability to withstand high copper concentrations.