Combining Biomimetic Block Copolymer Worms with an Ice‐Inhibiting Polymer for the Solvent‐Free Cryopreservation of Red Blood Cells. (28th January 2016)

• Record Type: Journal Article
• Authors: Mitchell, Daniel E.; Lovett, Joseph R.; Armes, Steven P.; Gibson, Matthew I.
• Is Part Of: Angewandte Chemie. Volume 128:Number 8(2016)
• Page Start: 2851
• Page End: 2854
• Publication Date: 2016-01-28
• Subjects: Biomaterialien -- Blockcopolymere -- Kryokonservierung -- Micellen -- Polymere; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201511454
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The first fully synthetic polymer‐based approach for red‐blood‐cell cryopreservation without the need for any (toxic) organic solvents is reported. Highly hydroxylated block copolymer worms are shown to be a suitable replacement for hydroxyethyl starch as a extracellular matrix for red blood cells. When used alone, the worms are not a particularly effective preservative. However, when combined with poly(vinyl alcohol), a known ice‐recrystallization inhibitor, a remarkable additive cryopreservative effect is observed that matches the performance of hydroxyethyl starch. Moreover, these block copolymer worms enable post‐thaw gelation by simply warming to 20 °C. This approach offers a new solution for both the storage and transport of red blood cells and also a convenient matrix for subsequent 3D cell cultures.