Vitamin D inhibits lipopolysaccharide-induced inflammatory response potentially through the Toll-like receptor 4 signalling pathway in the intestine and enterocytes of juvenile Jian carp (Cyprinus carpio var. Jian). Issue 10 (8th September 2015)

• Record Type: Journal Article
• Authors: Jiang, Jun; Shi, Dan; Zhou, Xiao-Qiu; Yin, Long; Feng, Lin; Jiang, Wei-Dan; Liu, Yang; Tang, Ling; Wu, Pei; Zhao, Ye
• Is Part Of: British journal of nutrition. Volume 114:Issue 10(2015)
• Page Start: 1560
• Page End: 1568
• Publication Date: 2015-09-08
• Subjects: Lipopolysaccharides, -- Enterocytes, -- Cyprinus carpio var. Jian, -- Toll-like receptor 4-myeloid differentiation primary response gene 88 signalling pathway; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114515003256
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: The present study was conducted to investigate the anti-inflammatory effect of vitamin D both in juvenile Jian carp ( Cyprinus carpio var. Jian) in vivo and in enterocytes in vitro . In primary enterocytes, exposure to 10 mg lipopolysaccharide (LPS)/l increased lactate dehydrogenase activity in the culture medium ( P <0·05) and resulted in a significant loss of cell viability ( P <0·05). LPS exposure increased ( P <0·05) the mRNA expression of pro-inflammatory cytokines (TNF- α, IL-1 β, IL-6 and IL-8), which was decreased by pre-treatment with 1, 25-dihydroxyvitamin D (1, 25D3) in a dose-dependent manner ( P <0·05). Further results showed that pre-treatment with 1, 25D3 down-regulated Toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (Myd88) and NF- κ B p65 mRNA expression ( P <0·05), suggesting potential mechanisms against LPS-induced inflammatory response. In vivo, intraperitoneal injection of LPS significantly increased TNF- α, IL-1 β, IL-6 and IL-8 mRNA expression in the intestine of carp ( P <0·05). Pre-treatment of fish with vitamin D3 protected the fish intestine from the LPS-induced increase of TNF- α, IL-1 β, IL-6 and IL-8 mainly by downregulating TLR4, Myd88 and NF- κ B p65 mRNA expression ( P <0·05). These observations suggest that vitamin D could inhibit LPS-induced inflammatory response in juvenile Jian carp in vivo and in enterocytes in vitro . The anti-inflammatory effect of vitamin D is mediated at least in part by TLR4-Myd88 signalling pathways in the intestine and enterocytes of juvenile Jian carp.