Isolation of a Clostridium acetobutylicum strain and characterization of its fermentation performance on agricultural wastes. (February 2016)

• Record Type: Journal Article
• Authors: Al-Shorgani, Najeeb Kaid Nasser; Isa, Mohd Hafez Mohd; Yusoff, Wan Mohtar Wan; Kalil, Mohd Sahaid; Hamid, Aidil Abdul
• Is Part Of: Renewable energy. Volume 86(2016)
• Page Start: 459
• Page End: 465
• Publication Date: 2016-02
• Subjects: Isolation -- Characterization -- Biobutanol -- Clostridium -- Renewable energy; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2015.08.051
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: A new solvent-producing Clostridium has been isolated from soil used in intensive rice cultivation. The 16S rRNA analysis of the isolate indicates that it is closely related to Clostridium acetobutylicum, with a sequence identity of 96%. The new isolate, named C. acetobutylicum YM1, produces biobutanol from multiple carbon sources, including glucose, fructose, xylose, arabinose, glycerol, lactose, cellobiose, mannitol, maltose, galactose, sucrose and mannose. This isolate can also utilize polysaccharides such as starch and carboxylmethyl cellulose (CMC) for the production of biobutanol. The ability of isolate YM1 to produce biobutanol from agro-industrial wastes was also evaluated for rice bran, de-oiled rice bran, palm oil mill effluent and palm kernel cake. The highest concentration of biobutanol (7.27 g/L) was obtained from the fermentation medium containing 2% (w/v) fructose, with a total acetone–butanol–ethanol (ABE) concentration of 10.23 g/L. The ability of isolate YM1 to produce biobutanol from various carbon sources and agro-wastes indicates the promise of the use of this isolate for the production of biobutanol, a renewable energy resource, from readily available renewable feedstocks. Highlights: A new wild-type of Clostridium acetobutylicum strain YM1 was isolated and characterized. Strain YM1 is capable of utilizing various carbon sources for biobutanol production. Strain YM1 is also capable of utilizing agroindustrial wastes for biobutanol production. Strain YM1 has amylolytic and cellulolytic activities.