Quantitative nanoscale electrostatics of viruses. Issue 41 (31st July 2015)

• Record Type: Journal Article
• Authors: Hernando-Pérez, M.; Cartagena-Rivera, A. X.; Lošdorfer Božič, A.; Carrillo, P. J. P.; San Martín, C.; Mateu, M. G.; Raman, A.; Podgornik, R.; de Pablo, P. J.
• Is Part Of: Nanoscale. Volume 7:Issue 41(2015)
• Page Start: 17289
• Page End: 17298
• Publication Date: 2015-07-31
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c5nr04274g
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : The recognition events between viruses and host cells are dominated by both specific and non-specific electrostatic interactions determined by the charge of viral particles. Here we probe the charge of individual viruses in aqueous solutions by measuring the electrostatic force between each viral particle and the Atomic Force Microscope tip. Abstract : Electrostatics is one of the fundamental driving forces of the interaction between biomolecules in solution. In particular, the recognition events between viruses and host cells are dominated by both specific and non-specific interactions and the electric charge of viral particles determines the electrostatic force component of the latter. Here we probe the charge of individual viruses in liquid milieu by measuring the electrostatic force between a viral particle and the Atomic Force Microscope tip. The force spectroscopy data of co-adsorbed ϕ29 bacteriophage proheads and mature virions, adenovirus and minute virus of mice capsids is utilized for obtaining the corresponding density of charge for each virus. The systematic differences of the density of charge between the viral particles are consistent with the theoretical predictions obtained from X-ray structural data. Our results show that the density of charge is a distinguishing characteristic of each virus, depending crucially on the nature of the viral capsid and the presence/absence of the genetic material.