Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy. Issue 10 (20th July 2015)

• Record Type: Journal Article
• Authors: Mascaraque, Ainhoa; Kowalczyk, Wioleta; Fernández, Tahia; Palomares, Francisca; Mayorga, Cristobalina; Andreu, David; Rojo, Javier
• Is Part Of: MedChemComm. Volume 6:Issue 10(2015:Oct.)
• Page Start: 1755
• Page End: 1760
• Publication Date: 2015-07-20
• Subjects: Pharmaceutical chemistry -- Periodicals; 615.19
• DOI: 10.1039/c5md00133a
• Languages: English
• ISSNs: 2040-2503

Abstract

Abstract : A GDP displaying the NP366–374 influenza A virus epitope induces an immunostimulatory effect, emerging as a potential flu vaccine. Abstract : Mannosylation facilitates uptake and internalization of immunogenic peptides by antigen-processing cells expressing mannose receptors at their surface, such as DC-SIGN, a lectin that plays a key role in the immune response against different pathogens. This internalization, processing and subsequent MHC presentation may result in a strong T cell stimulation. Here, we hypothesized that combining mannose glycodendrons with multivalent presentation of peptide epitopes in a likewise dendron format would yield hybrid constructs, named glycodendropeptides (GDPs), with the capacity to enhance peptide immunogenicity, hence providing a novel and versatile platform for applications in immunotherapy. Thus, GDPs of different valencies displaying the NP366–374 epitope, a conserved sequence from the influenza A virus nucleoprotein (NP), have been built by two click chemistry -based methodologies and assessed as potential flu vaccine candidates. Preliminary evaluation of the ability of these constructs to stimulate dendritic cell maturation and lymphocyte proliferation was promising, showing the highest-functionalized NP366–374 GDPs as inducing the strongest immunostimulatory effect.