Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation. Issue 97 (14th October 2015)

• Record Type: Journal Article
• Authors: Cao, Yuan; Nguyen, Giang K. T.; Tam, James P.; Liu, Chuan-Fa
• Is Part Of: Chemical communications. Volume 51:Issue 97(2015)
• Page Start: 17289
• Page End: 17292
• Publication Date: 2015-10-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc07227a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Recombinant proteins with a C-terminal thioester are readily prepared through the catalysis of butelase 1 – a powerful peptide ligase. Abstract : Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.