Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine. Issue 88 (21st September 2015)

• Record Type: Journal Article
• Authors: Abdelkader, E. H.; Feintuch, A.; Yao, X.; Adams, L. A.; Aurelio, L.; Graham, B.; Goldfarb, D.; Otting, G.
• Is Part Of: Chemical communications. Volume 51:Issue 88(2015)
• Page Start: 15898
• Page End: 15901
• Publication Date: 2015-09-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc07121f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : First example of gadolinium tags attached to a pair of unnatural amino acids for distance measurements by double electron–electron resonance. Abstract : Quantitative cysteine-independent ligation of a Gd 3+ tag to genetically encoded p -azido-l -phenylalanine via Cu(i )-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd 3+ –Gd 3+ distance measurements by double electron–electron resonance (DEER) experiments, as the position of the Gd 3+ ion relative to the protein can be predicted with high accuracy.