Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(iii) complex. Issue 89 (24th September 2015)

• Record Type: Journal Article
• Authors: Lin, Sheng; He, Bingyong; Yang, Chao; Leung, Chung-Hang; Mergny, Jean-Louis; Ma, Dik-Lung
• Is Part Of: Chemical communications. Volume 51:Issue 89(2015)
• Page Start: 16033
• Page End: 16036
• Publication Date: 2015-09-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc06655g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A novel luminescent G-quadruplex-selective iridium(iii ) complex was employed in a G-quadruplex-based detection assay for IFN-γ. Abstract : In this study, we synthesized a series of 9 luminescent iridium(iii ) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii ) complex 8 [Ir(pbtz)2 (dtbpy)]PF6 (where pbtz = 2-phenylbenzo[ d ]thiazole; dtbpy = 4, 4′-di- tert -butyl-2, 2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.