Click synthesis of boronic acid-functionalized molecularly imprinted silica nanoparticles with polydopamine coating for enrichment of trace glycoproteins. Issue 23 (11th November 2015)

• Record Type: Journal Article
• Authors: Sun, Lixiang; Lin, Danhong; Lin, Guowei; Wang, Ling; Lin, Zian
• Is Part Of: Analytical methods. Volume 7:Issue 23(2015)
• Page Start: 10026
• Page End: 10031
• Publication Date: 2015-11-11
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay02131f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Novel boronic acid-functionalized molecularly imprinted silica nanoparticles with polydopamine coating were prepared and applied to the recognition and enrichment of glycoproteins. Abstract : A facile strategy based on the synergistic effect of molecular imprinting and boronate affinity was proposed for glycoprotein imprinting. Polydopamine (PDA)-coated boronic acid-functionalized molecularly imprinted silica nanoparticles (MIPs) were prepared by the "thiol–ene" click reaction using SiO2 as the core, 3-acrylamidophenyl boronic acid (AAPBA) as the functional monomer, and horseradish peroxidase (HRP) as the glycoprotein template. A well defined core–shell structure of MIPs was obtained after self-polymerization of dopamine (DA) on the surface of HRP-immobilized silica nanoparticles (NPs). The polymerization conditions and adsorption behavior were investigated in detail in order to obtain the highest selectivity and binding capacity. Under the optimized conditions, the HRP-MIPs showed higher binding affinity towards HRP than non-imprinted nanoparticles (NIPs), and the corresponding adsorption capacity ( Q ) and imprinted factor ( α ) reached 0.58 μmol g −1 and 2.6, respectively. The specificity for HRP recognition was evaluated with a competitive experiment, and the results indicated that the HRP-MIPs had higher selectivity for the template. The good features of the HRP-MIPs facilitated selective isolation and enrichment of trace HRP from human serum. In addition, the stability and regeneration were also investigated, which indicated that the HRP-MIPs had excellent reusability.