Using the inherent chemistry of the endothelin-1 peptide to develop a rapid assay for pre-transplant donor lung assessment. Issue 24 (9th November 2015)

• Record Type: Journal Article
• Authors: Sage, Andrew T.; Bai, Xiaohui; Cypel, Marcelo; Liu, Mingyao; Keshavjee, Shaf; Kelley, Shana O.
• Is Part Of: Analyst. Volume 140:Issue 24(2015)
• Page Start: 8092
• Page End: 8096
• Publication Date: 2015-11-09
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an01536g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : An assay for the lung transplant biomarker ET-1 is reported that allows levels of this molecule to be reported with record-breaking speed and sensitivity. Abstract : Endothelin-1 is a potent vasoconstrictive peptide that plays an important role in ex vivo lung perfusion. ET-1 expression levels are predictive of lung transplant outcomes and represent a valuable monitoring tool for surgeons; however, traditional techniques that measure [ET-1] are not suitable for the transplant setting. Herein, we demonstrate a new assay that rapidly measures ET-1 peptide levels in lung perfusate.