Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection. Issue 43 (6th October 2015)

• Record Type: Journal Article
• Authors: Reverte, Maëva; Vasseur, Jean-Jacques; Smietana, Michael
• Is Part Of: Organic & biomolecular chemistry. Volume 13:Issue 43(2015)
• Page Start: 10604
• Page End: 10608
• Publication Date: 2015-10-06
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c5ob01815c
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Boronic acid modified DNA emerged as a new class of resistant oligonucleotides against enzymatic degradation. This property has been used to develop an enzyme-assisted label free method for mismatch detection. Abstract : 5′-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.