The oxidation of p-phenylenediamine, an ingredient used for permanent hair dyeing purposes, leads to the formation of hydroxyl radicals: Oxidative stress and DNA damage in human immortalized keratinocytes. Issue 3 (15th December 2015)

• Record Type: Journal Article
• Authors: Zanoni, Thalita B.; Hudari, Felipe; Munnia, Armelle; Peluso, Marco; Godschalk, Roger W.; Zanoni, Maria Valnice B.; den Hartog, Gertjan J.M.; Bast, Aalt; Barros, Silvia B.M.; Maria-Engler, Silvya S.; Hageman, Geja J.; de Oliveira, Danielle Palma
• Is Part Of: Toxicology letters. Volume 239:Issue 3(2015)
• Page Start: 194
• Page End: 204
• Publication Date: 2015-12-15
• Subjects: p-Phenylenediamine -- Hair dyes -- HaCaT -- Oxidative stress -- DNA damage; Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2015.09.026
• Languages: English
• ISSNs: 0378-4274

Abstract

Graphical abstract: Abstract: The hair-dyeing ingredient, p -phenylenediamine (PPD), was previously reported to be mutagenic, possibly by inducing oxidative stress. However, the exact mechanism of PPD in inducing oxidative stress upon skin exposure during hair-dyeing in human keratinocytes remains unknown. The aim of our studies was therefore to investigate the toxicity of PPD and its by-products in human immortalized keratinocytes (HaCaT) after auto-oxidation and after reaction with hydrogen peroxide (H2 O2 ). We found that the PPD half maximal effective cytotoxic concentration (EC50 ) to HaCaT is 39.37 and 35.63 μg/mL after 24 and 48 h, respectively, without addition of H2 O2 to induce oxidation. When PPD (10 or 100 μg/mL) is combined with 10.5 μg/mL of H2 O2, intracellular ROS production by HaCaT after 1 h was significantly increased and enhanced levels of DNA damage were observed after 4 h of exposure. After 24 h incubations, 20 μg/mL of PPD increased the level of DNA oxidation in HaCaT. Also, we found that the in vitro reaction between PPD and H2 O2, even below the maximum allowance by cosmetic industries, released hydroxyl radicals which can damage DNA. Taken together, we conclude that PPD alone and when combined with H2 O2 increases the formation of reactive oxygen species in human keratinocytes, leading to oxidative stress and subsequent DNA damage. These alterations suggest that the mechanism by which PPD exposure, alone or combined with H2 O2, damages keratinocytes by the formation of the high reactive HO radicals.