Inhibition of Yap2 activity by MAPKAP kinase Rck1 affects yeast tolerance to cadmium. Issue 19 (18th August 2015)

• Record Type: Journal Article
• Authors: Mazzola, Daiane; Pimentel, Catarina; Caetano, Soraia; Amaral, Catarina; Menezes, Regina; Santos, Claudia N.; Eleutherio, Elis; Rodrigues-Pousada, Claudina
• Is Part Of: FEBS letters. Volume 589:Issue 19(2015) Part B
• Page Start: 2841
• Page End: 2849
• Publication Date: 2015-08-18
• Subjects: Rck1 -- Yap2 -- Cadmium -- MAPK-activated protein -- Yeast; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.07.049
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Yap2 is a cadmium responsive transcription factor that interacts with MAPK‐activated protein (MAPKAP) kinase Rck1. We show that Rck1 deletion confers protection against cadmium toxicity and that the mechanism underlying this observation relies on Yap2. Rck1 removal from the yeast genome potentiates Yap2 activity by increasing protein half‐life and delaying its nuclear export. As a consequence, several Yap2 antioxidant targets are over‐activated by a mechanism that also requires Yap1. Several genes of the cell wall integrity (CWI) pathway are upregulated under cadmium stress in a Yap2 dependent way. We showed that deletion of CWI genes renders yeast cells more sensitive to cadmium. These findings led us to suggest that in response to cadmium stress Yap2 may serve a dual purpose: oxidative stress attenuation and cell wall maintenance. Abstract : The absence of Rck1 confers protection against cadmium toxicity. Yap2 mediates rck1 mutant resistance to cadmium. Rck1 affects Yap2 activity and interferes with its stability and localization. Yap2 together with Yap1 mediates the induction of antioxidant genes observed in the rck1 mutant. Under cadmium stress CWI genes are induced in a Yap2, but not Yap1, dependent manner.