Low‐intensity pulsed ultrasound‐induced ATP increases bone formation via the P2X7 receptor in osteoblast‐like MC3T3‐E1 cells. Issue 3 (24th December 2014)

• Record Type: Journal Article
• Authors: Manaka, Soichiro; Tanabe, Natsuko; Kariya, Taro; Naito, Masako; Takayama, Tadahiro; Nagao, Mayu; Liu, Di; Ito, Koichi; Maeno, Masao; Suzuki, Naoto; Miyazaki, Masashi
• Is Part Of: FEBS letters. Volume 589:Issue 3(2015)
• Page Start: 310
• Page End: 318
• Publication Date: 2014-12-24
• Subjects: Low-intensity pulsed ultrasound -- Adenosine triphosphate -- P2X7 -- Osteogenesis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.12.013
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Low‐intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS‐induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS‐induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS‐induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase. Abstract : LIPUS induces extracellular ATP release. LIPUS induces osteoblasts differentiation factors via P2X7. LIPUS promotes bone formation via P2X7 independently of ALPase activity.