MicroRNA‐134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins. Issue 2 (17th December 2014)

• Record Type: Journal Article
• Authors: Gao, Yongtao; Liu, Te; Huang, Yongyi
• Is Part Of: FEBS letters. Volume 589:Issue 2(2015)
• Page Start: 207
• Page End: 214
• Publication Date: 2014-12-17
• Subjects: Human endometrial cancer stem cell -- MicroRNA-134 -- DLK1-DIO3 genomic imprinted microRNA cluster -- Protein O-glucosyltransferase 1 -- Notch pathway -- Proliferation -- Migration; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.12.002
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : We aimed to ascertain the role of microRNAs (miRNAs) in regulating human endometrial cancer stem cells (HuECSCs). The expression level of miRNA‐134 (miR‐134), a member of the DLK1‐DIO3 genomic imprinted miRNA cluster, differed significantly between HuECSCs and human endometrial cancer cells (HuECCs). miR‐134 inhibited HuECSCs proliferation and migration by targeting protein O ‐glucosyltransferase 1 (POGLUT1) expression. Exogenous miR‐134 overexpression downregulated POGLUT1 and Notch pathway proteins in HuECSCs in vitro. miR‐134 overexpression affected the G2/M phase of HuECSCs and suppressed the growth of xenograft tumours formed. Thus, endogenous miR‐134 regulation in HuECSCs may suppress tumourigenesis in human endometrial carcinoma. Abstract : We isolated human endometrial cancer stem cells from endometrial cancer tissues. Low miRNA‐134 in HuECSCs correlated with POGLUT1 overexpression. We confirmed POGLUT1 mRNA to be a novel target of miRNA‐134. miR‐134 overexpression specifically inhibited HuECSCs proliferation and migration. miR‐134 overexpression suppressed Notch‐1 pathway in HuECSCs.