Fructose promotes the differentiation of 3T3‐L1 adipocytes and accelerates lipid metabolism. Issue 3 (24th December 2013)

• Record Type: Journal Article
• Authors: Legeza, Balázs; Balázs, Zoltán; Odermatt, Alex
• Is Part Of: FEBS letters. Volume 588:Issue 3(2014)
• Page Start: 490
• Page End: 496
• Publication Date: 2013-12-24
• Subjects: 11β-hydroxysteroid dehydrogenase -- Glucocorticoid -- Adipocyte -- Lipolysis -- Metabolic syndrome -- Fructose -- Metabolism -- 11β-HSD -- 11β-hydroxysteroid dehydrogensae -- ATGL -- adipocyte triglyceride lipase -- C/EBPα -- CCAT/enhancer-binding protein α -- DMEM -- Dulbecco's modified Eagles medium -- ER -- endoplasmic reticulum -- F6P -- fructose-6-phosphate -- FBS -- fetal bovine serum -- FFA -- free fatty acids -- GLUT5 -- glucose transporter 5 -- HPRT -- hypoxanthine-guanine phosphoribosyltransferase -- H6PDH -- hexose-6-phosphate dehydrogenase -- HSL -- hormone sensitive lipase -- PPARγ -- peroxisome proliferator-activated receptor γ -- TLC -- thin layer chromatography; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.12.014
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Excessive fructose consumption and elevated glucocorticoids contribute to metabolic syndrome. We show that fructose as the only carbohydrate source is sufficient for the differentiation of 3T3‐L1 fibroblasts into adipocytes. Differentiation of cells in fructose containing medium resulted in increased 11β‐hydroxysteroid dehydrogenase 1 (11β‐HSD1) expression and activity. Experiments with transfected HEK‐293 cells suggested more efficient NADPH generation by fructose compared with glucose in the endoplasmic reticulum (ER). Adipocytes differentiated in the presence of fructose showed increased FABP4 expression, C/EBPα to C/EBPβ ratio and lipolysis. Thus, excessive fructose may cause adverse metabolic effects by enhancing 11β‐HSD1 activity and increasing lipolysis in adipocytes. Abstract : Fructose is a potent adipocyte differentiation agent. Fructose stimulates NADPH generation in the endoplasmic reticulum. Fructose enhances glucocorticoid activation in adipocytes. Enhanced glucocorticoid activation increases lipolysis.