Erythroid differentiation is augmented in Reelin‐deficient K562 cells and homozygous reeler mice. Issue 1 (12th November 2013)

• Record Type: Journal Article
• Authors: Chu, Hui-Chun; Lee, Hsing-Ying; Huang, Yen-Shu; Tseng, Wei-Lien; Yen, Ching-Ju; Cheng, Ju-Chien; Tseng, Ching-Ping
• Is Part Of: FEBS letters. Volume 588:Issue 1(2014)
• Page Start: 58
• Page End: 64
• Publication Date: 2013-11-12
• Subjects: EPO -- erythropoietin -- FBS -- fetal bovine serum -- Hct -- hematocrit -- Hb -- hemoglobin -- HRP -- horseradish peroxidase -- MCH -- mean corpuscular hemoglobin -- MCHC -- mean corpuscular hemoglobin concentration -- MCV -- mean corpuscular volume -- NaB -- sodium butyrate -- NFAT -- nuclear factor of activated T cells -- PAGE -- polyacrylamide gel electrophoresis -- PBS -- phosphate-buffered saline -- PVDF -- polyvinylidene fluoride -- RDW -- red blood cell distribution width -- RPMI -- Roswell Park Memorial Institute -- SDS -- sodium dodecyl sulfate -- TMB -- 3, 3′, 5, 5′-tetramethylbenzidine -- Erythroid differentiation -- K562 cell -- Sodium butyrate -- Reeler mice -- Reelin; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.11.002
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up‐regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119 + CD71 + erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined. Abstract : We identify a function of Reelin in erythroid cells. Reelin is up‐regulated during erythroid differentiation of K562 cells. Deficient Reelin expression augments erythroid differentiation. Reelin‐deficient reeler mice display typical polycythemia characteristics. Reelin plays a negative regulatory role in erythroid differentiation.