Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis. Issue 2 (2nd November 2015)

• Record Type: Journal Article
• Authors: Ikeda, Atsuko; Kajiwara, Kentaro; Iwamoto, Kunihiko; Makino, Asami; Kobayashi, Toshihide; Mizuta, Keiko; Funato, Kouichi
• Is Part Of: Yeast. Volume 33:Issue 2(2016:Feb.)
• Page Start: 37
• Page End: 42
• Publication Date: 2015-11-02
• Subjects: human ARV1 -- GPI anchor -- complementation -- arv1Δ mutant -- yeast; Yeast -- Periodicals; Yeasts -- Periodicals; Yeasts -- genetics -- Periodicals; Electronic journals; 547
• DOI: 10.1002/yea.3138
• Languages: English
• ISSNs: 0749-503X

Abstract

Abstract: ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae . Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1 Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans. Copyright © 2015 John Wiley & Sons, Ltd.