Programmable bio-nano-chip system: a flexible point-of-care platform for bioscience and clinical measurements. Issue 20 (26th August 2015)

• Record Type: Journal Article
• Authors: McRae, Michael P.; Simmons, Glennon W.; Wong, Jorge; Shadfan, Basil; Gopalkrishnan, Sanjiv; Christodoulides, Nicolaos; McDevitt, John T.
• Is Part Of: Lab on a chip. Volume 15:Issue 20(2015)
• Page Start: 4020
• Page End: 4031
• Publication Date: 2015-08-26
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c5lc00636h
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : The programmable bio-nano-chip (p-BNC) is an ultra-flexible system for multiplexed and multiclass assays on a universal modular lab-on-a-chip platform for clinical and bioscience applications at the point-of-care. Abstract : The development of integrated instrumentation for universal bioassay systems serves as a key goal for the lab-on-a-chip community. The programmable bio-nano-chip (p-BNC) system is a versatile multiplexed and multiclass chemical- and bio-sensing system for bioscience and clinical measurements. The system is comprised of two main components, a disposable cartridge and a portable analyzer. The customizable single-use plastic cartridges, which now can be manufactured in high volumes using injection molding, are designed for analytical performance, ease of use, reproducibility, and low cost. These labcard devices implement high surface area nano-structured biomarker capture elements that enable high performance signaling and are index-matched to real-world biological specimens. This detection modality, along with the convenience of on-chip fluid storage in blisters and self-contained waste, represents a standard process to digitize biological signatures at the point-of-care. A companion portable analyzer prototype has been developed to integrate fluid motivation, optical detection, and automated data analysis, and it serves as the human interface for complete assay automation. In this report, we provide a systems-level perspective of the p-BNC universal biosensing platform with an emphasis on flow control, device integration, and automation. To demonstrate the flexibility of the p-BNC, we distinguish diseased and non-case patients across three significant disease applications: prostate cancer, ovarian cancer, and acute myocardial infarction. Progress towards developing a rapid 7 minute myoglobin assay is presented using the fully automated p-BNC system.